Beyond Rg: BioSAXS Short Course 2015

July 6 - 9, 2015
Advanced Photon Source, Argonne National Laboratory

Course Photo (1.2Mb jpeg)

Course Program (pdf)

SAS imagesSolution small-angle x-ray scattering has earned great popularity and success in biological studies owing to the advances in brilliant x-ray sources, large area detectors, and, more importantly, data analysis methods in the past two decades. The objective of the “Beyond Rg: BioSAXS Short Course 2015” is to raise the awareness and capabilities of the small-angle scattering (SAS) techniques in the structural biology community by providing an intermediate-level course for those in need of a better understanding of bioSAS theory, and techniques and facilities provided at the APS.

This bioSAXS short course offers an overview of small-angle scattering (SAS, including x-ray and neutron scattering) theory, SAS methods and capabilities for structural biology, and data reduction and analysis tools, to enable the community to submit highly effective beam-time proposals and to facilitate better utilization of the resources at the APS. Although many methods covered in this course were originally developed for biomolecules which are often mono-dispersed, this course may be also useful for those work with solution samples with high structural homogeneity.

The course includes lectures, software practice, and hands-on experimental sessions. Experimental session will be carried out at selected APS small-angle x-ray scattering beamlines for samples that are brought by participants and demo samples. Example data will be provided for software practice. Lecturers will be available during software practice and experimental sessions for one-on-one discussion.

Participants are expected to have attained at least a doctoral-level education and are encouraged to have a defined experimental program so that they can collect data for a set of samples that they bring. Participants are also encouraged to bring their own notebook computers.

Registration fee: $50
Number of participants is limited to 30-35. If needed, organizers reserve the right to select participants.

Registration closed:  

Participants will be responsible for their own transportation, accommodations, and meal costs. A limited number of rooms has been reserved at the Argonne Guest House:

Argonne Guest House
Argonne National Laboratory
9700 S. Cass Avenue - Building 460
Argonne, IL 60439
Phone: 800.632.8990 or 630.739.6000
Fax: 630.739.1000

Participants are responsible for reserving these rooms.

For answers to questions or for more information contact:

Rachel Reed
Argonne National Laboratory
9700 S. Cass Ave., Bldg. 401
Argonne, IL U.S.A. or


APS X-ray science division


July 6, 8-9, 2015 General lectures (theory, applications, software practice, etc.)
July 7, 2015 Optional experiments at 3 selected SAXS beamlines



Fundamentals of Small-Angle Scattering
Theories and Applications of BioSAXS
BioSAXS Sample Preparation
Small Angle Scattering Instrumentation
Specific Topics of BioSAXS Methods on Various Systems
Time-Resolved X-ray Scattering
Software Practice
Strategies to Write Successful Beam-Time Proposals


Srinivas Chakravarthy, BioCAT/IIT
Jan Ilavsky, XSD/ Argonne
Tom Irving, BioCAT/IIT
Irina Kosheleva, BioCARS/U Chicago
Soenke Seifert, XSD/Argonne
Weifeng Shang, BioCAT/IIT
Steven Weigand, DND-CAT/Northwestern
Xiaobing Zuo, XSD/Argonne
and other APS XSD and APS CAT's staff members



12-ID-B (XSD,
14-ID (Bio-CARS,
18-ID-D (Bio-CAT,

Details on the beamlines:




From bottom to top: The bird view of the Advanced Photon Source (APS) of Argonne National Laboratory; SAXS/WAXS instruments at beamline 12-ID-B of APS; Scattering image of a solution sample; Superimposition of SAXS bead model and atomic models of a RNA molecule.